Journal: iScience

Article Title: Islet cell stress induced by insulin-degrading enzyme deficiency promotes regeneration and protection from autoimmune diabetes

doi: 10.1016/j.isci.2024.109929

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-ATF6 , Novus Biologicals , Cat# NBP1-40256; RRID: AB_2058774.

Techniques: Recombinant, Lysis, Protease Inhibitor, Staining, Plasmid Preparation, Blocking Assay, Reverse Transcription, SYBR Green Assay, Imaging, Mass Spectrometry, Enzyme-linked Immunosorbent Assay, Software, Transmission Assay, Microscopy

Journal: Life Science Alliance

Article Title: Nr1h4 and Thrb ameliorate ER stress and provide protection in the MPTP mouse model of Parkinson’s

doi: 10.26508/lsa.202302416

Figure Lengend Snippet: (A, B, C) qRT–PCR analysis of ER stress genes in control and (A) Nr1h4 knockdown, (B) Thrb knockdown, or (C) Nr1h4 and Thrb double-knockdown differentiated N2a cells, treated with TM, in the presence or absence of GW4064 or T3 or both. (D, E, F) Immunoblot analysis of all the ER stress markers involved in three UPR pathways (IRE, PERK, and ATF6) in control and (D) Nr1h4 knockdown, (E) Thrb knockdown, or (F) Nr1h4 and Thrb double-knockdown differentiated N2a cells, treated with TM, in the presence or absence of GW4064 or T3 or both. Data are the mean ± SD or representative of three independent experiments performed in triplicates. * P <= 0.05, ** P < 0.01, *** P < 0.001 versus control group or as indicated by a two-tailed t test.

Article Snippet: Primary antibodies used were as follows: BiP (C50B12) (#3177; CST), CHOP (L63F7) (#2895; CST), PERK (#5683 D11A8; CST), phospho-PERK (Thr980) (16F8) (#3179; CST), eIF2α (#9722; CST), phospho-eIF2α (Ser51) (#9721; CST), ATF-4 (D4B8) (#11815; CST), ATF6 (70B1413.1) (#NBP1-40256SS; Novus Biologicals), IRE1α (14C10) (#3294; CST), phospho-IRE1α (pSer724) (#NB100-2323SS; Novus Biologicals), XBP1 (#NBP1-77681SS; Novus Biologicals), tyrosine hydroxylase (E2L6M) (#58844; CST), Bax (D2E11) (#5023; CST), cleaved PARP1 (E51) (#ab32064; Abcam), Mcl-1 (D35A5) (#5453; CST), Bcl-2 (50E3; CST), farnesoid X receptor (D-3) (#sc-25309; SCBT), Thrb (#ab5622; Abcam), PPARγ (E−8) (#sc-7273; SCBT), β-actin (C4) (#sc-47778; SCBT).

Techniques: Quantitative RT-PCR, Control, Knockdown, Western Blot, Two Tailed Test

Journal: Zoological Research

Article Title: ATF6 aggravates apoptosis in early porcine embryonic development by regulating organelle homeostasis under high-temperature conditions

doi: 10.24272/j.issn.2095-8137.2023.080

Figure Lengend Snippet: Effects of high-temperature (HT) on ATF6 expression pattern and localization

Article Snippet: Embryos were then incubated at 4 °C overnight with primary antibodies against ATF6 (1:100; Novus Biologicals, NBP1-40256, USA), AIFM2 (1:100; Proteintech, 20886-1-AP, China), and giantin (1:100; Abcam, ab80864, UK).

Techniques: Expressing

Journal: Zoological Research

Article Title: ATF6 aggravates apoptosis in early porcine embryonic development by regulating organelle homeostasis under high-temperature conditions

doi: 10.24272/j.issn.2095-8137.2023.080

Figure Lengend Snippet: Effects of ATF6 knockdown on high-temperature (HT)-embryonic development

Article Snippet: Embryos were then incubated at 4 °C overnight with primary antibodies against ATF6 (1:100; Novus Biologicals, NBP1-40256, USA), AIFM2 (1:100; Proteintech, 20886-1-AP, China), and giantin (1:100; Abcam, ab80864, UK).

Techniques: Knockdown

Journal: Zoological Research

Article Title: ATF6 aggravates apoptosis in early porcine embryonic development by regulating organelle homeostasis under high-temperature conditions

doi: 10.24272/j.issn.2095-8137.2023.080

Figure Lengend Snippet: Effects of ATF6 knockdown on ER function in high-temperature (HT)-embryos

Article Snippet: Embryos were then incubated at 4 °C overnight with primary antibodies against ATF6 (1:100; Novus Biologicals, NBP1-40256, USA), AIFM2 (1:100; Proteintech, 20886-1-AP, China), and giantin (1:100; Abcam, ab80864, UK).

Techniques: Knockdown

Journal: Zoological Research

Article Title: ATF6 aggravates apoptosis in early porcine embryonic development by regulating organelle homeostasis under high-temperature conditions

doi: 10.24272/j.issn.2095-8137.2023.080

Figure Lengend Snippet: Effects of ATF6 knockdown on Golgi function in high-temperature (HT)-embryos

Article Snippet: Embryos were then incubated at 4 °C overnight with primary antibodies against ATF6 (1:100; Novus Biologicals, NBP1-40256, USA), AIFM2 (1:100; Proteintech, 20886-1-AP, China), and giantin (1:100; Abcam, ab80864, UK).

Techniques: Knockdown

Journal: Zoological Research

Article Title: ATF6 aggravates apoptosis in early porcine embryonic development by regulating organelle homeostasis under high-temperature conditions

doi: 10.24272/j.issn.2095-8137.2023.080

Figure Lengend Snippet: Effects of ATF6 on mitochondrial function and apoptosis in high-temperature (HT)-embryos

Article Snippet: Embryos were then incubated at 4 °C overnight with primary antibodies against ATF6 (1:100; Novus Biologicals, NBP1-40256, USA), AIFM2 (1:100; Proteintech, 20886-1-AP, China), and giantin (1:100; Abcam, ab80864, UK).

Techniques:

Journal: Zoological Research

Article Title: ATF6 aggravates apoptosis in early porcine embryonic development by regulating organelle homeostasis under high-temperature conditions

doi: 10.24272/j.issn.2095-8137.2023.080

Figure Lengend Snippet: Mechanistic representation of ATF6-mediated exacerbation of apoptosis in early porcine embryonic development through regulation of organelle homeostasis under HT conditions

Article Snippet: Embryos were then incubated at 4 °C overnight with primary antibodies against ATF6 (1:100; Novus Biologicals, NBP1-40256, USA), AIFM2 (1:100; Proteintech, 20886-1-AP, China), and giantin (1:100; Abcam, ab80864, UK).

Techniques: